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Published date: 13 May 2024
Open opportunity - This means that the contract is currently active, and the buying department is looking for potential suppliers to fulfil the contract.
Research services - 73110000
London
£167,000
BIP861438760
13 May 2024
13 June 2024
12pm
29 July 2024
29 July 2025
Service contract
Open procedure
Any interested supplier may submit a tender in response to an opportunity notice.
Yes
No
Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp.
In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306.
RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.
Links
Follow the instructions given in the description or the more information section.
The Herbarium, Kew Green
Richmond,
London
TW9 3AE
UK
+442083325000
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